求翻译成英文
Bombyx mori Densovirus (Bombyx two Mori bidensovirus, BmBDV) is the first to have its encoding DNA polymerase invertebrate viruses, its genome consists of two kinds of ssDNA (VD1 and VD2). The virus specific recognition of the host cell is a columnar epithelial cells of the midgut of silkworm, silkworm pathogens causing chronic is concentrated nuclear conditions, its replication mechanism is not clear. This paper mainly in the BmN and Sf9 insect cells, the full-length genome fragments of the virus of two kinds of linear co transfection of these two kinds of insect cells, and the transfected cells of silkworm larvae feeding back a sense of susceptible strain, respectively from the viral gene transcription and expression in insect cells, to detection of transfected to produce infectious virus particles, and thus to replication of the virus to speculate and study. 1, the linear co transfected cells were infected by testing and training methods to explore the enzyme the virus genome cloning plasmid pT-VD1 and pUC-VD2, precipitation collection with a specific enzyme cutting site genome fragments VD1 and VD2 using alcohol, linearization were transfected into BmN cells, the cultured and continuous cultivation in two ways, the cultured for 20 days. (1) to collect the cells and supernatant medium as template, PCR was detected, amplified bands of virus gene into cultured in continuous culture and passage of cell mass. (2) total protein extraction cells and supernatant were detected in Western blot, cultured cell clusters in the cross to the bands associated with viral proteins. (3) after transfected into cultured BmN cells were blown, indirect immunofluorescence, positive reaction was localized in the nucleus. Save the virus back 2, the BmN cells transfected BmN cell mass detection collected respectively, feed and susceptible strains of Bombyx mori in 306 of the two instar silkworm larvae, feeding to five age fourth days. (1) from the midgut of silkworm genome, as detected by PCR in BmN cells, feeding the genome can be amplified to stripe virus gene. (2) to extract the total protein of Bombyx mori midgut, through the Western blot detection, hybridization and virus gene expression bands relevant only when total protein feeding cell clusters in. 3, the linear co transfected Sf9 cell infection detection will digestion collection of genomic fragments VD1 and VD2, linear co transfection of BmN and Sf9 cells were cultured for 20 days. (1) the genome of transfected cells was, by demethylation treatment, PCR detection, can be amplified to stripe virus gene in BmN genome. (2) the total protein in transfected cells by Western extraction, detection of blot hybridization, the strip is associated with viral protein can only in the total protein in BmN cell. 4, to save the virus transfected Sf9 cells to sense the transfected collection of BmN and Sf9 cells, respectively, feed and susceptible strains of Bombyx mori Jingsong two instar silkworm larvae, feeding to five age fourth days. (1) extract the midgut tissues of the genome, as detected by PCR, only in the feed and strip virus gene amplified from BmN cells group. (2) to extract the total protein of Bombyx mori midgut tissues by Western blot hybridization, detection, the strip is associated with viral protein can only in the total protein of BmN cells in feed. Therefore, the method of genome segment linearization BmBDV were transfected into BmN cells under in vitro conditions, can save the infectious virus particles. Keywords: Silkworm two densonucleosis virus, BmN cells, Sf9 cells, linear co transfection, virus rescue
My consideration is based on the results of an issue and the effect it will exert towards my friends.
First things first, someone must give the first priority to the results when taking an issue into account. The lack of thinking before our leap will bring unnecessary trouble for us. And then we need to look at the big picture in our friends´shoes. Always supposing that we refuse to do so will result in the issue undone and the lose of our friends.
1 】in the manager under the guidance of the timely completion of 80 enterprises, accounting for tax filing 2 】hard-working, have a strong sense of responsibility, able to work with high intensity 3 】participate in * * * special audit certificate access link 4 】proactive, highly learning ability, to master a variety of office article member 5 】participating organizations related to electronic commerce knowledge lectures, responsible for the guidance of teachers and students in order to advance and retreat 6 】to participate in the "Webpage design competition", won the team third 7 】participate in the organization of industrial and commercial administration department "love life, I smile" creative t-shirt design contest, as shown in the final model into T-shirt 8 】participating in the Department of business administration "mother's Day" Challenge Cup "activities, responsible for you" game, and is responsible for the process of social practice with the racket, recording a group of final team
1.Under the guidance of manager unluckily complete 80 companies do zhang declare dutiable goods work
2.Bears hardships and stands hard work, have a strong sense of responsibility, can adapt to high work intensity
3.Participate in query link * * * special audit certificate
4, proactive, have a high ability to learn, to master all kinds of clerical work of the office
5. Participate in organize lecture on e-commerce knowledge, responsible for guide the orderly into exits between teachers and students
6. Take part in the campus "web design competition", to third place team
7. To participate in the organization department of business administration "love life, I smile" creative T-shirt design contest, as a display model for final t-shirts
8. To participate in the organization department of business administration "challenge cup" mother's day activity, is responsible for the "dripping of grace" link, and is responsible for the team with pats, record a set of final process of social practice
1 in the manager under the guidance of the timely completion of 80 enterprises, accounting for tax filing
2 hard-working, have a strong sense of responsibility, able to work with high intensity
3 participate in * * * special audit certificate access link
4, proactive, highly learning ability, to master a variety of office article member
5 participating organizations related to electronic commerce knowledge lectures, responsible for the guidance of teachers and students in order to advance and retreat
6 to participate in the "Webpage design competition", won the team third
7 participate in the organization of industrial and commercial administration department "love life, I smile" creative t-shirt design contest, as shown in the final model into T-shirt
8 participating in the Department of business administration "mother's Day" Challenge Cup "activities, responsible for you" game, and is responsible for the process of social practice with the racket, recording a group of final team
求英文翻译成中文
答：To be a better one 想为你把自己变完美 to satisfy you 就为了让你满意 for you’re everything to me 因为你就是我的一切 And I'll do what you ask me 我愿为你做一切 If you let me be free 只要你让我感觉自由自在 If you want me 如果你想要和我在一起 satisfy me 就请让我快...
求中文翻译为英文
答：Do dump the garbage into the trashbin, we are responsible for protecting the environment and watch the hygiene.3、红灯停，绿灯行，黄灯亮了等一等，我们要遵守交通规则。Stop in Red, move in Green, and wait in Yellow, we've got to obey the traffic rules.4、爱护别人的劳动成果，...
求翻译成英文,在线等。
答：You should focus the most basic part when your learning English:to recite more glossary,to read more grammer,to say more and to listen more.Then you will like it gradually.
求翻译成英文
答：I wish （that) it were none other than me / it were no other than me that / who bear / stand / endure loneliness until the death comes.（指现在情况的虚拟语气）I wish (that) it had been none other than me / it had been no other than me that / who bore / stood ...
求翻译成英文
答：of local area network on basic-level radio and television and its security solution.4 信息隐藏算法的研究 4.The research on hiding information algorithm 楼上翻译得很好，但是“实现”在这里似乎更应该用implement。implement可用于项目的实现，而realization更偏向于主观上的计划，梦想之类的实现。
求翻译成英文
答：I am a special examinee, I know, my poor : 1,. English listening is very poor, the basic will not hear. Therefore, I hope you can use Chinese to answer some question. 2. I is a professional, so, I started the foundation is very bad, but this does not affect, I ...
求翻译成英语
答：at the same time, it offers a variety of options, to meet the different needs of individuals, families, and promote the formation of a healthy lifestyle. The company will make annual adjustment to basic salary structure, ensure thesame industry accounted for competitive advantage.
求翻译成英文
答：最后就到你该去的窗口排队吧。Behind every window sits a visa officer, or serious or kind, both in Chinese and English bars, two language freedom, against will not work.每个玻璃窗后面坐着一位签证官，或严肃或和蔼，中英文俱棒，两种语言转换自由，不服都不行。
求翻译成英文
答：there is a feeling, that is emptiness; I like to walk alone, tree-lined road, when marching with a foot floating mottled light, when the "rustling" sound blew my face, there is a feeling, it is ethereal Vacant, take me into my world. There is always a moment of illusion...
求翻译成英语
答：将中文译成英语 She still dare to use the knowledge she learned about their own views and analysis, and some very unique and detailed view, indicating that she was not satisfied with the facile mind of the knowledge, but rather the pursuit of a mastery of knowledge and ...
